Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3385Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 48ng/ml | 2.18 | 2.159 | 2.097 |
| 2.138 | |||
| 24ng/ml | 1.424 | 1.44 | 1.378 |
| 1.456 | |||
| 12ng/ml | 0.897 | 0.877 | 0.815 |
| 0.858 | |||
| 6ng/ml | 0.457 | 0.496 | 0.434 |
| 0.536 | |||
| 3ng/ml | 0.256 | 0.273 | 0.211 |
| 0.29 | |||
| 0ng/ml | 0.057 | 0.062 | 0 |
| 0.067 |
Precision
We measured random samples of E3385Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.15 | 0.49 | 5.98 |
| Intra-Assay | 2 | 18 | 28.99 | 1.07 | 3.69 |
| Intra-Assay | 3 | 18 | 12.58 | 0.71 | 5.65 |
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