Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3412Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 6.4ng/ml | 2.514 | 2.583 | 2.532 |
| 2.652 | |||
| 3.2ng/ml | 1.505 | 1.521 | 1.47 |
| 1.537 | |||
| 1.6ng/ml | 0.873 | 0.844 | 0.793 |
| 0.814 | |||
| 0.8ng/ml | 0.456 | 0.431 | 0.38 |
| 0.407 | |||
| 0.4ng/ml | 0.207 | 0.202 | 0.151 |
| 0.197 | |||
| 0ng/ml | 0.035 | 0.051 | 0 |
| 0.066 |
Precision
We measured random samples of E3412Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 4 | 0.14 | 3.38 |
| Intra-Assay | 2 | 18 | 1.69 | 0.08 | 4.78 |
| Intra-Assay | 3 | 18 | 0.78 | 0.04 | 5.15 |
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