Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3485Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 480ng/mL | 2.116 | 2.242 | 2.204 |
| 2.368 | |||
| 240ng/mL | 1.734 | 1.619 | 1.581 |
| 1.503 | |||
| 120ng/mL | 1.185 | 1.104 | 1.066 |
| 1.024 | |||
| 60ng/mL | 0.685 | 0.728 | 0.69 |
| 0.772 | |||
| 30ng/mL | 0.448 | 0.446 | 0.408 |
| 0.445 | |||
| 0ng/mL | 0.048 | 0.038 | 0 |
| 0.029 |
Precision
We measured random samples of E3485Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 197.21 | 8.69 | 4.4 |
| Intra-Assay | 2 | 18 | 31.66 | 2.04 | 6.43 |
| Intra-Assay | 3 | 18 | 119.31 | 7.6 | 6.37 |
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