Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3489Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 32ng/ml | 2.31 | 2.307 | 2.251 |
| 2.304 | |||
| 16ng/ml | 1.743 | 1.817 | 1.761 |
| 1.89 | |||
| 8ng/ml | 1.111 | 1.151 | 1.095 |
| 1.191 | |||
| 4ng/ml | 0.627 | 0.651 | 0.595 |
| 0.675 | |||
| 2ng/ml | 0.426 | 0.404 | 0.348 |
| 0.381 | |||
| 0ng/ml | 0.053 | 0.056 | 0 |
| 0.058 |
Precision
We measured random samples of E3489Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3.71 | 0.19 | 5 |
| Intra-Assay | 2 | 18 | 16.28 | 0.9 | 5.53 |
| Intra-Assay | 3 | 18 | 6.24 | 0.35 | 5.62 |
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