Human Rapamycin Insensitive Companion of Mtor, RICTOR ELISA Kit

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Home > Product > ELISA Kit > Human Rapamycin Insensitive Companion of Mtor, RICTOR ELISA Kit

Human Rapamycin Insensitive Companion of Mtor, RICTOR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rapamycin insensitive companion of mTOR

Code

E3592Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.

UniProt accession

Q6R327

MASS（Da）

192,218

GeneID

253260

Synonyms

AVO3;AVO3 homolog;hAVO3;PIA;Rapamycin-insensitive companion of mTOR;RICTOR

Gene names

RICTOR

Research area

Cell Biology

Target protein

RICTOR

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E3592Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.155	2.241	2.191
	2.327
4ng/ml	1.327	1.353	1.303
	1.379
2ng/ml	0.792	0.78	0.731
	0.769
1ng/ml	0.486	0.47	0.421
	0.455
0.5ng/ml	0.288	0.274	0.225
	0.26
0ng/ml	0.04	0.05	0
	0.059

Precision

We measured random samples of E3592Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.5	0.16	4.48
Intra-Assay	2	18	4.77	0.14	3.03
Intra-Assay	3	18	1.77	0.11	6.44

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E3592Hu

$320.00
96T

E3592Hu

$458.00

Add to Cart

Lead time: Within two weeks

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