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Home > Product > ELISA Kit > Human Polycomb Complex Protein BMI-1, BMI1 ELISA Kit

Human Polycomb Complex Protein BMI-1, BMI1 ELISA Kit

Human Polycomb Complex Protein BMI-1, BMI1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.99ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Polycomb complex protein BMI-1

Code

E3604Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.99ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:15386022, PubMed:16359901, PubMed:26151332, PubMed:16714294, PubMed:21772249, PubMed:25355358, PubMed:27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed:21772249, PubMed:25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed:15386022, PubMed:26151332, PubMed:21772249).

UniProt accession

MASS(Da)

36,949

GeneID

648

Synonyms

BMI1;FLVI2/BMI1;flvi-2/bmi-1;PCGF4;Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51;RNF51

Gene names

BMI1

Research area

Others

Target protein

BMI1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3604Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.379 2.294 2.26
2.209
800ng/L 1.739 1.753 1.718
1.767
400ng/L 1.232 1.221 1.187
1.21
200ng/L 0.631 0.631 0.597
0.632
100ng/L 0.349 0.381 0.346
0.412
0ng/L 0.037 0.034 0
0.031

Precision

We measured random samples of E3604Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 379.13 21.34 5.63
Intra-Assay 2 18 542.09 23.83 4.4
Intra-Assay 3 18 183.81 9.51 5.18

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3604Hu

    $320.00

  • 96T

    E3604Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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