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Home > Product > ELISA Kit > Human Atp Synthase Subunit Beta, Mitochondrial, ATP5B ELISA Kit

Human Atp Synthase Subunit Beta, Mitochondrial, ATP5B ELISA Kit

Human Atp Synthase Subunit Beta, Mitochondrial, ATP5B ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.32ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.32ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

ATP Synthase subunit beta, mitochondrial

Code

E3614Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.32ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

UniProt accession

MASS(Da)

56,560

GeneID

506

Synonyms

ATP Synthase F1 subunit beta;ATP Synthase subunit beta, mitochondrial;ATP5B;ATPMB;ATPSB;HEL-S-271

Gene names

ATP5F1B

Research area

Tags & Cell Markers

Target protein

ATP5B

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3614Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.496 2.418 2.369
2.34
800ng/L 1.566 1.493 1.444
1.42
400ng/L 0.831 0.79 0.741
0.75
200ng/L 0.447 0.435 0.386
0.424
100ng/L 0.26 0.259 0.21
0.259
0ng/L 0.054 0.049 0
0.045

Precision

We measured random samples of E3614Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 581.14 30.88 5.31
Intra-Assay 2 18 864.85 29.79 3.44
Intra-Assay 3 18 160.07 9.31 5.81

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3614Hu

    $320.00

  • 96T

    E3614Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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