Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E3626Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.187 | 2.22 | 2.165 |
| 2.252 | |||
| 12ng/ml | 1.887 | 1.829 | 1.775 |
| 1.771 | |||
| 6ng/ml | 1.208 | 1.147 | 1.093 |
| 1.086 | |||
| 3ng/ml | 0.657 | 0.66 | 0.606 |
| 0.663 | |||
| 1.5ng/ml | 0.359 | 0.379 | 0.325 |
| 0.4 | |||
| 0ng/ml | 0.053 | 0.054 | 0 |
| 0.056 |
Precision
We measured random samples of E3626Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 5.65 | 0.37 | 6.49 |
| Intra-Assay | 2 | 18 | 8.73 | 0.38 | 4.32 |
| Intra-Assay | 3 | 18 | 3.07 | 0.17 | 5.59 |
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