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Home > Product > ELISA Kit > Human Serine Threonine-protein Kinase B-raf, BRAF ELISA Kit

Human Serine Threonine-protein Kinase B-raf, BRAF ELISA Kit

Human Serine Threonine-protein Kinase B-raf, BRAF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.11ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

serine/threonine-protein kinase B-raf

Code

E3906Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.11ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

UniProt accession

MASS(Da)

84,437

GeneID

673

Synonyms

BRAF;B-raf;BRAF1;B-RAF1;NS7;p94;Proto-oncogene B-Raf;RAFB1;Serine/threonine-protein kinase B-raf;v-Raf murine sarcoma viral oncogene homolog B1

Gene names

BRAF

Research area

Cell Biology

Target protein

BRAF

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3906Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.171 2.287 2.231
2.404
1200ng/L 1.313 1.352 1.296
1.392
600ng/L 0.832 0.8 0.744
0.769
300ng/L 0.487 0.474 0.418
0.462
150ng/L 0.314 0.319 0.263
0.325
0ng/L 0.073 0.056 0
0.04

Precision

We measured random samples of E3906Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1170.07 42 3.59
Intra-Assay 2 18 491.29 39.47 8.03
Intra-Assay 3 18 980.84 53.92 5.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3906Hu

    $320.00

  • 96T

    E3906Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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