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Home > Product > ELISA Kit > Human Arf-Gap with Sh3 Domain, Ank Repeat and Ph Domain-Coutaining Protein 1, ASAP1 ELISA Kit

Human Arf-Gap with Sh3 Domain, Ank Repeat and Ph Domain-Coutaining Protein 1, ASAP1 ELISA Kit

Human Arf-Gap with Sh3 Domain, Ank Repeat and Ph Domain-Coutaining Protein 1, ASAP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.91ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.91ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Arf-GAP with SH3 Domain,ANK repeat and PH Domain-Coutaining Protein 1

Code

E4128hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.91ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis.

UniProt accession

MASS(Da)

125,498

GeneID

50807

Synonyms

130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein;ADP-ribosylation factor-directed GTPase-activating protein 1;AMAP1;ARF GTPase-activating protein 1;Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1;ASAP1;CENTB4;DDEF1;DEF-1;Development and differentiation-enhancing factor 1;Differentiation-enhancing factor 1;PAG2;PAP;PIP2-dependent ARF1 GAP;ZG14P

Gene names

ASAP1

Research area

Tags & Cell Markers

Target protein

ASAP1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Arf-Gap with Sh3 Domain, Ank Repeat and Ph Domain-Coutaining Protein 1, ASAP1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.179 2.189 2.133
2.199
1200ng/L 1.177 1.213 1.157
1.249
600ng/L 0.805 0.717 0.661
0.63
300ng/L 0.437 0.429 0.373
0.421
150ng/L 0.175 0.176 0.12
0.177
0ng/L 0.063 0.056 0
0.049

Precision

We measured random samples of E4128hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 503.37 35.35 7.02
Intra-Assay 2 18 1085.03 60.84 5.61
Intra-Assay 3 18 1662.64 48.75 2.93

Protocols

References

Customer reviews and Q&As

No reviews
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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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