Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4314hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2000ng/L | 2.166 | 2.241 | 2.19 |
| 2.315 | |||
| 1000ng/L | 1.501 | 1.408 | 1.358 |
| 1.316 | |||
| 500ng/L | 0.838 | 0.799 | 0.748 |
| 0.759 | |||
| 250ng/L | 0.41 | 0.419 | 0.368 |
| 0.427 | |||
| 125ng/L | 0.288 | 0.264 | 0.214 |
| 0.241 | |||
| 0ng/L | 0.065 | 0.05 | 0 |
| 0.035 |
Precision
We measured random samples of E4314hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1317.58 | 52.6 | 3.99 |
| Intra-Assay | 2 | 18 | 332.18 | 19.56 | 5.89 |
| Intra-Assay | 3 | 18 | 521.05 | 27.4 | 5.26 |
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