Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4866Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.31 | 2.213 | 2.17 |
| 2.116 | |||
| 1200ng/L | 1.714 | 1.713 | 1.67 |
| 1.713 | |||
| 600ng/L | 1.225 | 1.237 | 1.194 |
| 1.249 | |||
| 300ng/L | 0.735 | 0.668 | 0.625 |
| 0.6 | |||
| 150ng/L | 0.359 | 0.369 | 0.326 |
| 0.378 | |||
| 0ng/L | 0.054 | 0.043 | 0 |
| 0.032 |
Precision
We measured random samples of E4866Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 708.78 | 32.21 | 4.54 |
| Intra-Assay | 2 | 18 | 382.89 | 16.93 | 4.42 |
| Intra-Assay | 3 | 18 | 176.58 | 10.78 | 6.1 |
Protocols
References
Customer reviews and Q&As
