Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4876Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.043 | 2.207 | 2.173 |
| 2.371 | |||
| 600ng/L | 1.172 | 1.267 | 1.233 |
| 1.361 | |||
| 300ng/L | 0.632 | 0.696 | 0.662 |
| 0.76 | |||
| 150ng/L | 0.505 | 0.51 | 0.476 |
| 0.516 | |||
| 75ng/L | 0.223 | 0.211 | 0.178 |
| 0.199 | |||
| 0ng/L | 0.041 | 0.034 | 0 |
| 0.027 |
Precision
We measured random samples of E4876Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 909.71 | 33 | 3.63 |
| Intra-Assay | 2 | 18 | 388.46 | 20.33 | 5.23 |
| Intra-Assay | 3 | 18 | 135.9 | 9.05 | 6.66 |
Protocols
References
Customer reviews and Q&As
