Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4906Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 240ng/mL | 2.083 | 2.109 | 2.057 |
| 2.135 | |||
| 120ng/mL | 1.153 | 1.148 | 1.095 |
| 1.143 | |||
| 60ng/mL | 0.806 | 0.725 | 0.673 |
| 0.644 | |||
| 30ng/mL | 0.468 | 0.439 | 0.387 |
| 0.411 | |||
| 15ng/mL | 0.191 | 0.208 | 0.155 |
| 0.225 | |||
| 0ng/mL | 0.048 | 0.053 | 0 |
| 0.057 |
Precision
We measured random samples of E4906Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 182.33 | 5.48 | 3 |
| Intra-Assay | 2 | 18 | 65.99 | 4.36 | 6.61 |
| Intra-Assay | 3 | 18 | 110.11 | 5.02 | 4.56 |
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