Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4948Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4800ng/L | 2.186 | 2.179 | 2.136 |
| 2.172 | |||
| 2400ng/L | 1.399 | 1.409 | 1.367 |
| 1.42 | |||
| 1200ng/L | 0.79 | 0.778 | 0.735 |
| 0.765 | |||
| 600ng/L | 0.426 | 0.43 | 0.387 |
| 0.435 | |||
| 300ng/L | 0.203 | 0.189 | 0.146 |
| 0.174 | |||
| 0ng/L | 0.051 | 0.043 | 0 |
| 0.034 |
Precision
We measured random samples of E4948Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2207.41 | 63.33 | 2.87 |
| Intra-Assay | 2 | 18 | 1675.5 | 99.74 | 5.95 |
| Intra-Assay | 3 | 18 | 684.45 | 30.19 | 4.41 |
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