Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E4955Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 1.993 | 1.868 | 1.832 |
| 1.744 | |||
| 800ng/L | 1.027 | 0.992 | 0.956 |
| 0.957 | |||
| 400ng/L | 0.467 | 0.536 | 0.499 |
| 0.605 | |||
| 200ng/L | 0.271 | 0.224 | 0.188 |
| 0.177 | |||
| 100ng/L | 0.152 | 0.146 | 0.11 |
| 0.14 | |||
| 0ng/L | 0.027 | 0.036 | 0 |
| 0.046 |
Precision
We measured random samples of E4955Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 342.79 | 19.85 | 5.79 |
| Intra-Assay | 2 | 18 | 1513.31 | 50.56 | 3.34 |
| Intra-Assay | 3 | 18 | 711.64 | 38.6 | 5.42 |
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