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Home > Product > ELISA Kit > Human Apoptosis Regulator Bax, BAX ELISA Kit

Human Apoptosis Regulator Bax, BAX ELISA Kit

Human Apoptosis Regulator Bax, BAX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.26ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Apoptosis regulator BAX

Code

E4977Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.26ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

UniProt accession

MASS(Da)

21,184

GeneID

581

Synonyms

Apoptosis regulator BAX;BAX;BCL2L4;Bcl2-L-4;Bcl-2-like protein 4

Gene names

BAX

Research area

Cell Biology

Target protein

BAX

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E4977Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.189 2.205 2.158
2.222
40ng/ml 1.202 1.226 1.179
1.25
20ng/ml 0.664 0.666 0.619
0.668
10ng/ml 0.518 0.505 0.458
0.491
5ng/ml 0.214 0.205 0.158
0.197
0ng/ml 0.057 0.047 0
0.036

Precision

We measured random samples of E4977Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 13.27 0.82 6.16
Intra-Assay 2 18 43.94 1.31 2.97
Intra-Assay 3 18 33.24 2.01 6.05

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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