Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5005Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 2.084 | 2.066 | 2.036 |
| 2.047 | |||
| 4ng/ml | 0.968 | 0.977 | 0.947 |
| 0.986 | |||
| 2ng/ml | 0.402 | 0.501 | 0.472 |
| 0.601 | |||
| 1ng/ml | 0.183 | 0.179 | 0.15 |
| 0.176 | |||
| 0.5ng/ml | 0.165 | 0.155 | 0.125 |
| 0.145 | |||
| 0ng/ml | 0.027 | 0.03 | 0 |
| 0.032 |
Precision
We measured random samples of E5005Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1.63 | 0.07 | 4.3 |
| Intra-Assay | 2 | 18 | 7 | 0.19 | 2.74 |
| Intra-Assay | 3 | 18 | 4.86 | 0.22 | 4.45 |
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