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Home > Product > ELISA Kit > Human Cdk5 Regulatory Subunit-associated Protein 2, CDK5RAP2 ELISA Kit

Human Cdk5 Regulatory Subunit-associated Protein 2, CDK5RAP2 ELISA Kit

Human Cdk5 Regulatory Subunit-associated Protein 2, CDK5RAP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.19ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

CDK5 regulatory subunit-associated protein 2

Code

E5025Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.19ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion. Involved in regulation of mitotic spindle orientation (By similarity). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Together with EB1/MAPRE1, may promote microtubule polymerization, bundle formation, growth and dynamics at the plus ends. Regulates centrosomal maturation by recruitment of the gamma-tubulin ring complex (gamma-TuRC) onto centrosomes (PubMed:26485573). In complex with PDE4DIP isoform 13/MMG8/SMYLE, MAPRE1 and AKAP9, contributes to microtubules nucleation and extension from the centrosome to the cell periphery (PubMed:29162697). Required for the recruitment of AKAP9 to centrosomes (PubMed:29162697). Plays a role in neurogenesis (By similarity).

UniProt accession

MASS(Da)

215,038

GeneID

55755

Synonyms

C48;CDK5 activator-binding protein C48;CDK5 regulatory subunit-associated protein 2;CDK5RAP2;Centrosome-associated protein 215;Cep215;MCPH3

Gene names

CDK5RAP2

Research area

Cell Biology

Target protein

CDK5RAP2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5025Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.575 2.587 2.554
2.599
600ng/L 1.566 1.542 1.509
1.518
300ng/L 0.883 0.86 0.827
0.838
150ng/L 0.384 0.387 0.354
0.391
75ng/L 0.282 0.241 0.208
0.201
0ng/L 0.027 0.033 0
0.04

Precision

We measured random samples of E5025Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 171.44 9.92 5.79
Intra-Assay 2 18 615.99 20.49 3.33
Intra-Assay 3 18 268.14 10.36 3.86

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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