Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5050Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.114	2.223	2.164
	2.332
6ng/ml	1.268	1.289	1.23
	1.309
3ng/ml	0.793	0.753	0.694
	0.712
1.5ng/ml	0.394	0.43	0.372
	0.467
0.75ng/ml	0.222	0.213	0.155
	0.204
0ng/ml	0.059	0.059	0
	0.058

We measured random samples of E5050Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.35	0.13	5.63
Intra-Assay	2	18	4.82	0.28	5.75
Intra-Assay	3	18	7.97	0.32	3.96