Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5050Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.114 | 2.223 | 2.164 |
| 2.332 | |||
| 6ng/ml | 1.268 | 1.289 | 1.23 |
| 1.309 | |||
| 3ng/ml | 0.793 | 0.753 | 0.694 |
| 0.712 | |||
| 1.5ng/ml | 0.394 | 0.43 | 0.372 |
| 0.467 | |||
| 0.75ng/ml | 0.222 | 0.213 | 0.155 |
| 0.204 | |||
| 0ng/ml | 0.059 | 0.059 | 0 |
| 0.058 |
Precision
We measured random samples of E5050Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 2.35 | 0.13 | 5.63 |
| Intra-Assay | 2 | 18 | 4.82 | 0.28 | 5.75 |
| Intra-Assay | 3 | 18 | 7.97 | 0.32 | 3.96 |
Protocols
References
Customer reviews and Q&As
Associated products
