Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5058Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 1.776 | 1.769 | 1.722 |
| 1.763 | |||
| 600ng/L | 1.059 | 1.087 | 1.04 |
| 1.116 | |||
| 300ng/L | 0.51 | 0.543 | 0.496 |
| 0.577 | |||
| 150ng/L | 0.184 | 0.222 | 0.174 |
| 0.259 | |||
| 75ng/L | 0.104 | 0.111 | 0.063 |
| 0.118 | |||
| 0ng/L | 0.048 | 0.048 | 0 |
| 0.047 |
Precision
We measured random samples of E5058Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1002.89 | 49.44 | 4.93 |
| Intra-Assay | 2 | 18 | 602.89 | 24.11 | 4 |
| Intra-Assay | 3 | 18 | 286.15 | 13.5 | 4.72 |
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