Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5091Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.277	2.213	2.159
	2.15
24ng/mL	1.225	1.224	1.17
	1.224
12ng/mL	0.792	0.808	0.754
	0.824
6ng/mL	0.526	0.516	0.461
	0.506
3ng/mL	0.212	0.209	0.155
	0.207
0ng/mL	0.057	0.054	0
	0.052

We measured random samples of E5091Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	26.9	0.82	3.05
Intra-Assay	2	18	19	1.13	5.94
Intra-Assay	3	18	9.74	0.62	6.42