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Home > Product > ELISA Kit > Human 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Human 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Human 14-3-3 Protein Epsilon, YWHAE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 14.77ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 14.77ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

14-3-3 Protein Epsilon

Code

E3713Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

14.77ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner (By similarity). Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm (PubMed: 12917326).

UniProt accession

MASS(Da)

29,174

GeneID

7531

Synonyms

14-3-3 protein epsilon;14-3-3E;YWHAE

Gene names

YWHAE

Research area

Immunology

Target protein

YWHAE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3713Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.246 2.271 2.237
2.296
2400ng/L 1.488 1.498 1.464
1.508
1200ng/L 0.7 0.648 0.614
0.595
600ng/L 0.304 0.325 0.291
0.345
300ng/L 0.142 0.179 0.145
0.216
0ng/L 0.025 0.034 0
0.042

Precision

We measured random samples of E3713Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2370 81.23 3.4
Intra-Assay 2 18 1429.9 54.74 3.8
Intra-Assay 3 18 724.9 34.16 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3713Hu

    $320.00

  • 96T

    E3713Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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