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Home > Product > ELISA Kit > Human Transmembrane Glycoprotein Nmb, GPNMB ELISA Kit

Human Transmembrane Glycoprotein Nmb, GPNMB ELISA Kit

Human Transmembrane Glycoprotein Nmb, GPNMB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-50ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.12ng/ml

Detection range: 0.2-50ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Transmembrane glycoprotein NMB

Code

E5111Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.12ng/ml

Detection range

0.2-50ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. Two transcript variants encoding 560 and 572 amino acid isoforms have been characterized for this gene in humans. The mouse and rat orthologues of GPNMB are known as DC-HIL and Osteoactivin (OA), respectively. GPNMB is a type I transmembrane glycoprotein which shows homology to the pmel17 precursor, a melanocyte-specific protein. In osteoblast progenitor cells, Osteoactivin works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization [9] that is mediated at least in part by Bone morphogenetic protein 2 in a SMAD1 dependent manner to promote osteoblast differentiation. In addition, using a rat fracture model, Osteoactivin (OA) enhances the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, Osteoactivin expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

UniProt accession

MASS(Da)

63,923

GeneID

10457

Synonyms

GPNMB;Hematopoietic growth factor inducible neurokinin-1 type;HGFIN;NMB;PLCA3;Transmembrane glycoprotein NMB

Gene names

GPNMB

Research area

Immunology

Target protein

GPNMB

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5111Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.428 2.404 2.359
2.379
16ng/ml 1.591 1.582 1.537
1.572
8ng/ml 0.94 0.913 0.868
0.885
4ng/ml 0.385 0.454 0.41
0.524
2ng/ml 0.253 0.24 0.196
0.228
0ng/ml 0.06 0.045 0
0.029

Precision

We measured random samples of E5111Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.54 0.43 5.06
Intra-Assay 2 18 13.97 0.4 2.88
Intra-Assay 3 18 5.83 0.31 5.28

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5111Hu

    $320.00

  • 96T

    E5111Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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