Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5130Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/mL | 2.276 | 2.217 | 2.169 |
| 2.158 | |||
| 12ng/mL | 1.386 | 1.462 | 1.414 |
| 1.537 | |||
| 6ng/mL | 0.935 | 0.861 | 0.813 |
| 0.787 | |||
| 3ng/mL | 0.472 | 0.47 | 0.422 |
| 0.468 | |||
| 1.5ng/mL | 0.236 | 0.265 | 0.217 |
| 0.294 | |||
| 0ng/mL | 0.044 | 0.048 | 0 |
| 0.052 |
Precision
We measured random samples of E5130Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 5.08 | 0.25 | 4.99 |
| Intra-Assay | 2 | 18 | 8.52 | 0.42 | 4.97 |
| Intra-Assay | 3 | 18 | 14.55 | 0.69 | 4.72 |
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