Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5169Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4800ng/L | 2.227 | 2.215 | 2.192 |
| 2.203 | |||
| 2400ng/L | 1.369 | 1.394 | 1.371 |
| 1.419 | |||
| 1200ng/L | 0.718 | 0.734 | 0.711 |
| 0.75 | |||
| 600ng/L | 0.407 | 0.417 | 0.394 |
| 0.426 | |||
| 300ng/L | 0.142 | 0.14 | 0.117 |
| 0.139 | |||
| 0ng/L | 0.022 | 0.023 | 0 |
| 0.023 |
Precision
We measured random samples of E5169Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1377.66 | 74.8 | 5.43 |
| Intra-Assay | 2 | 18 | 3167.91 | 112.99 | 3.57 |
| Intra-Assay | 3 | 18 | 996.38 | 51.33 | 5.15 |
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