Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5178Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.31 | 2.226 | 2.181 |
| 2.142 | |||
| 800ng/L | 1.332 | 1.289 | 1.244 |
| 1.245 | |||
| 400ng/L | 0.662 | 0.717 | 0.672 |
| 0.772 | |||
| 200ng/L | 0.445 | 0.435 | 0.39 |
| 0.424 | |||
| 100ng/L | 0.191 | 0.196 | 0.151 |
| 0.201 | |||
| 0ng/L | 0.06 | 0.045 | 0 |
| 0.03 |
Precision
We measured random samples of E5178Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 766.03 | 26 | 3.39 |
| Intra-Assay | 2 | 18 | 698.75 | 40.98 | 5.86 |
| Intra-Assay | 3 | 18 | 216.41 | 11.98 | 5.54 |
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