Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5214Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.291	2.167	2.125
	2.043
800ng/L	1.554	1.535	1.493
	1.515
400ng/L	0.751	0.665	0.623
	0.578
200ng/L	0.325	0.323	0.281
	0.32
100ng/L	0.202	0.208	0.167
	0.215
0ng/L	0.04	0.042	0
	0.044

We measured random samples of E5214Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	892.69	31.4	3.52
Intra-Assay	2	18	331.89	13.15	3.96
Intra-Assay	3	18	418.02	14.98	3.58