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Home > Product > ELISA Kit > Human Ectonucleoside Triphosphate Diphosphohydrolase 4, ENTPD4 ELISA Kit

Human Ectonucleoside Triphosphate Diphosphohydrolase 4, ENTPD4 ELISA Kit

Human Ectonucleoside Triphosphate Diphosphohydrolase 4, ENTPD4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.65ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.65ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ectonucleoside triphosphate diphosphohydrolase 4

Code

E5223Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.65ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the hydrolysis of nucleoside triphosphates and diphosphates in a calcium- or magnesium-dependent manner, with a preference for pyrimidines. Preferentially hydrolyzes UTP and TTP. AMP, ADP, ATP and UMP are not substrates (PubMed:10858452, PubMed:9556635). Preferentially activated by Ca2+ over Mg2+ (PubMed:10858452). Has a broad substrate specificity with the ability of cleaving all nucleotide di- and triphosphates with the exception of adenosine di- and triphosphate (ADP and ATP). Preferentially hydrolyzes CTP, UDP, CDP, GTP and GDP. Can use either Ca2+ or Mg2+ equally.

UniProt accession

MASS(Da)

70,255

GeneID

9583

Synonyms

Ectonucleoside triphosphate diphosphohydrolase 4;ENTPD4;Golgi UDPase;LALP70;LAP70;LYSAL1;Lysosomal apyrase-like protein of 70 kDa;NTPDase 4;NTPDase-4;UDPase;Uridine-diphosphatase

Gene names

ENTPD4

Research area

Epigenetics and Nuclear Signaling

Target protein

ENTPD4

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5223Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.13 2.113 2.05
2.095
600ng/L 1.265 1.274 1.212
1.283
300ng/L 0.815 0.788 0.726
0.76
150ng/L 0.463 0.451 0.389
0.44
75ng/L 0.234 0.207 0.145
0.181
0ng/L 0.062 0.062 0
0.062

Precision

We measured random samples of E5223Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 789.87 29.28 3.71
Intra-Assay 2 18 180.73 12.24 6.78
Intra-Assay 3 18 423.98 21.6 5.09

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5223Hu

    $320.00

  • 96T

    E5223Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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