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Home > Product > ELISA Kit > Human Elongation Factor 2, EEF2 ELISA Kit

Human Elongation Factor 2, EEF2 ELISA Kit

Human Elongation Factor 2, EEF2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.35ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.35ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

elongation factor 2

Code

E5231Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.35ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

UniProt accession

MASS(Da)

95,338

GeneID

1938

Synonyms

eEF2;EEF-2;EF2;EF-2;Elongation factor 2;SCA26

Gene names

EEF2

Research area

Epigenetics and Nuclear Signaling

Target protein

eEF2

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5231Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.153 2.101 2.053
2.049
800ng/L 1.56 1.617 1.568
1.674
400ng/L 1.066 1.034 0.985
1.001
200ng/L 0.77 0.777 0.728
0.784
100ng/L 0.441 0.455 0.407
0.469
0ng/L 0.064 0.049 0
0.033

Precision

We measured random samples of E5231Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 217.75 14.46 6.64
Intra-Assay 2 18 756.11 40.22 5.32
Intra-Assay 3 18 198.35 15.86 8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5231Hu

    $320.00

  • 96T

    E5231Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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