Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5276Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12.8ng/mL | 2.433 | 2.28 | 2.209 |
| 2.126 | |||
| 6.4ng/mL | 1.259 | 1.23 | 1.159 |
| 1.2 | |||
| 3.2ng/mL | 0.808 | 0.779 | 0.709 |
| 0.751 | |||
| 1.6ng/mL | 0.482 | 0.487 | 0.417 |
| 0.492 | |||
| 0.8ng/mL | 0.287 | 0.293 | 0.222 |
| 0.298 | |||
| 0ng/mL | 0.072 | 0.07 | 0 |
| 0.069 |
Precision
We measured random samples of E5276Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 6.64 | 0.21 | 3.23 |
| Intra-Assay | 2 | 18 | 1.47 | 0.1 | 6.99 |
| Intra-Assay | 3 | 18 | 5.22 | 0.31 | 5.87 |
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