Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5293Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.048 | 2.073 | 2.037 |
| 2.098 | |||
| 12ng/ml | 1.043 | 1.068 | 1.031 |
| 1.092 | |||
| 6ng/ml | 0.499 | 0.44 | 0.404 |
| 0.381 | |||
| 3ng/ml | 0.314 | 0.248 | 0.211 |
| 0.182 | |||
| 1.5ng/ml | 0.079 | 0.088 | 0.051 |
| 0.096 | |||
| 0ng/ml | 0.039 | 0.036 | 0 |
| 0.034 |
Precision
We measured random samples of E5293Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 10.82 | 0.47 | 4.33 |
| Intra-Assay | 2 | 18 | 19.89 | 0.54 | 2.7 |
| Intra-Assay | 3 | 18 | 8.36 | 0.28 | 3.4 |
Customer reviews and Q&As
Associated products