Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5306Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.117 | 2.155 | 2.104 |
| 2.192 | |||
| 600ng/L | 1.697 | 1.71 | 1.659 |
| 1.723 | |||
| 300ng/L | 1.275 | 1.223 | 1.172 |
| 1.171 | |||
| 150ng/L | 0.702 | 0.706 | 0.655 |
| 0.709 | |||
| 75ng/L | 0.441 | 0.399 | 0.349 |
| 0.358 | |||
| 0ng/L | 0.063 | 0.051 | 0 |
| 0.039 |
Precision
We measured random samples of E5306Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 238.35 | 17.9 | 7.51 |
| Intra-Assay | 2 | 18 | 397.72 | 20.03 | 5.04 |
| Intra-Assay | 3 | 18 | 91.11 | 5.53 | 6.07 |
Protocols
References
Customer reviews and Q&As
Associated products
