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Home > Product > ELISA Kit > Human G1 S-specific Cyclin-E1, CCNE1 ELISA Kit

Human G1 S-specific Cyclin-E1, CCNE1 ELISA Kit

Human G1 S-specific Cyclin-E1, CCNE1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.56ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

G1/S-specific cyclin-E1

Code

E5313Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.56ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

G1/S-specific cyclin-E1 is a protein that in humans is encoded by the CCNE1 gene. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.

UniProt accession

MASS(Da)

47,077

GeneID

898

Synonyms

CCNE;CCNE1;G1/S-specific cyclin-E1;pCCNE1

Gene names

CCNE1

Research area

Cell Biology

Target protein

CCNE1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human G1 S-specific Cyclin-E1, CCNE1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.195 2.149 2.091
2.102
800ng/L 1.452 1.365 1.306
1.277
400ng/L 0.811 0.831 0.772
0.85
200ng/L 0.525 0.518 0.46
0.511
100ng/L 0.272 0.263 0.205
0.254
0ng/L 0.075 0.058 0
0.042

Precision

We measured random samples of E5313Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 566.16 35.52 6.27
Intra-Assay 2 18 1000.23 36.77 3.68
Intra-Assay 3 18 317.84 23.92 7.53

Protocols

References

Customer reviews and Q&As

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Associated products

Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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