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Home > Product > ELISA Kit > Human Granzyme H, GZMH ELISA Kit

Human Granzyme H, GZMH ELISA Kit

Human Granzyme H, GZMH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.48ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.48ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Granzyme H

Code

E5359Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.48ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytotoxic chymotrypsin-like serine protease with preference for bulky and aromatic residues at the P1 position and acidic residues at the P3' and P4' sites. Probably necessary for target cell lysis in cell-mediated immune responses. Participates in the antiviral response via direct cleavage of several proteins essential for viral replication.

UniProt accession

MASS(Da)

27,315

GeneID

2999

Synonyms

Cathepsin G-like 2;CCP-X;CGL-2;CSP-C;CTLA1;CTSGL2;Cytotoxic serine protease C;Cytotoxic T-lymphocyte proteinase;Granzyme H;GZMH

Gene names

GZMH

Research area

Others

Target protein

GZMH

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5359Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.653 2.614 2.581
2.575
600ng/L 1.556 1.539 1.506
1.522
300ng/L 0.763 0.794 0.761
0.824
150ng/L 0.454 0.483 0.451
0.513
75ng/L 0.247 0.243 0.21
0.238
0ng/L 0.035 0.033 0
0.03

Precision

We measured random samples of E5359Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 665.86 23.48 3.53
Intra-Assay 2 18 166.51 8.07 4.85
Intra-Assay 3 18 357.74 15.67 4.38

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E5359Hu

    $320.00

  • 96T

    E5359Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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