Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5458Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1200ng/L | 2.357 | 2.205 | 2.164 |
| 2.053 | |||
| 600ng/L | 1.392 | 1.394 | 1.353 |
| 1.396 | |||
| 300ng/L | 0.791 | 0.822 | 0.781 |
| 0.852 | |||
| 150ng/L | 0.546 | 0.492 | 0.451 |
| 0.438 | |||
| 75ng/L | 0.22 | 0.252 | 0.211 |
| 0.283 | |||
| 0ng/L | 0.045 | 0.041 | 0 |
| 0.037 |
Precision
We measured random samples of E5458Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 606.76 | 23.64 | 3.9 |
| Intra-Assay | 2 | 18 | 218.82 | 12.99 | 5.94 |
| Intra-Assay | 3 | 18 | 288.7 | 17.83 | 6.18 |
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