Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5469Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.345 | 2.191 | 2.15 |
| 2.038 | |||
| 6ng/ml | 1.527 | 1.53 | 1.488 |
| 1.532 | |||
| 3ng/ml | 1.008 | 1.005 | 0.963 |
| 1.001 | |||
| 1.5ng/ml | 0.741 | 0.742 | 0.7 |
| 0.742 | |||
| 0.75ng/ml | 0.428 | 0.418 | 0.376 |
| 0.407 | |||
| 0ng/ml | 0.034 | 0.042 | 0 |
| 0.049 |
Precision
We measured random samples of E5469Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 0.83 | 0.06 | 7.53 |
| Intra-Assay | 2 | 18 | 7.54 | 0.37 | 4.85 |
| Intra-Assay | 3 | 18 | 3.5 | 0.29 | 8.29 |
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