Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Synonyms

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA plate	12 wells * 8 detachable strips
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

These standard curves of E5470Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.259	2.308	2.256
	2.357
24ng/mL	1.792	1.845	1.793
	1.898
12ng/mL	1.298	1.23	1.177
	1.162
6ng/mL	0.682	0.663	0.61
	0.643
3ng/mL	0.388	0.407	0.354
	0.426
0ng/mL	0.039	0.053	0
	0.066

We measured random samples of E5470Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.46	1.61	6.58
Intra-Assay	2	18	10.16	0.47	4.67
Intra-Assay	3	18	3.08	0.17	5.38