Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5492Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/mL | 2.366 | 2.378 | 2.317 |
| 2.39 | |||
| 60ng/mL | 1.394 | 1.394 | 1.332 |
| 1.393 | |||
| 30ng/mL | 0.833 | 0.85 | 0.789 |
| 0.867 | |||
| 15ng/mL | 0.442 | 0.435 | 0.374 |
| 0.427 | |||
| 7.5ng/mL | 0.255 | 0.264 | 0.203 |
| 0.274 | |||
| 0ng/mL | 0.069 | 0.061 | 0 |
| 0.053 |
Precision
We measured random samples of E5492Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 61.62 | 2.02 | 3.28 |
| Intra-Assay | 2 | 18 | 51.04 | 2.61 | 5.11 |
| Intra-Assay | 3 | 18 | 18.37 | 1.34 | 7.28 |
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