Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5501Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.169 | 2.237 | 2.195 |
| 2.305 | |||
| 1200ng/L | 1.178 | 1.286 | 1.245 |
| 1.394 | |||
| 600ng/L | 0.847 | 0.767 | 0.726 |
| 0.687 | |||
| 300ng/L | 0.523 | 0.473 | 0.432 |
| 0.423 | |||
| 150ng/L | 0.206 | 0.216 | 0.174 |
| 0.225 | |||
| 0ng/L | 0.058 | 0.041 | 0 |
| 0.025 |
Precision
We measured random samples of E5501Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1790.06 | 68 | 3.8 |
| Intra-Assay | 2 | 18 | 446.5 | 29.93 | 6.7 |
| Intra-Assay | 3 | 18 | 887.48 | 57.99 | 6.53 |
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