Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5541Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.189 | 2.207 | 2.169 |
| 2.225 | |||
| 800ng/L | 1.796 | 1.783 | 1.745 |
| 1.77 | |||
| 400ng/L | 1.12 | 1.128 | 1.09 |
| 1.137 | |||
| 200ng/L | 0.612 | 0.605 | 0.567 |
| 0.598 | |||
| 100ng/L | 0.405 | 0.413 | 0.375 |
| 0.42 | |||
| 0ng/L | 0.043 | 0.038 | 0 |
| 0.033 |
Precision
We measured random samples of E5541Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 854.2 | 40.17 | 4.7 |
| Intra-Assay | 2 | 18 | 177.1 | 8.69 | 4.91 |
| Intra-Assay | 3 | 18 | 345.41 | 20.76 | 6.01 |
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