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Home > Product > ELISA Kit > Human Cell Migration-inducing and Hyaluronan-binding Protein, CEMIP ELISA Kit

Human Cell Migration-inducing and Hyaluronan-binding Protein, CEMIP ELISA Kit

Human Cell Migration-inducing and Hyaluronan-binding Protein, CEMIP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.026ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cell migration-inducing and hyaluronan-binding protein

Code

E5542Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.026ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.

UniProt accession

MASS(Da)

152,998

GeneID

57214

Synonyms

CCSP1;Cell migration-inducing and hyaluronan-binding protein;HYBID;KIAA1199;TMEM2L

Gene names

CEMIP

Research area

Others

Target protein

KIAA1199

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E5542Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.072 2.105 2.044
2.138
6ng/ml 1.468 1.473 1.411
1.477
3ng/ml 0.754 0.782 0.721
0.811
1.5ng/ml 0.425 0.444 0.383
0.464
0.75ng/ml 0.25 0.241 0.18
0.233
0ng/ml 0.058 0.062 0
0.065

Precision

We measured random samples of E5542Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.37 0.25 3.93
Intra-Assay 2 18 1.31 0.07 5.17
Intra-Assay 3 18 4.24 0.27 6.25

Protocols

References

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E5542Hu

    $320.00

  • 96T

    E5542Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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