Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5548Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.272 | 2.252 | 2.204 |
| 2.233 | |||
| 1200ng/L | 1.36 | 1.422 | 1.374 |
| 1.484 | |||
| 600ng/L | 0.862 | 0.812 | 0.763 |
| 0.761 | |||
| 300ng/L | 0.466 | 0.504 | 0.455 |
| 0.542 | |||
| 150ng/L | 0.254 | 0.262 | 0.214 |
| 0.27 | |||
| 0ng/L | 0.058 | 0.048 | 0 |
| 0.039 |
Precision
We measured random samples of E5548Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1359.83 | 41.99 | 3.09 |
| Intra-Assay | 2 | 18 | 873.45 | 51.54 | 5.9 |
| Intra-Assay | 3 | 18 | 316.53 | 18.42 | 5.82 |
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