Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5589Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 1.894 | 1.834 | 1.792 |
| 1.775 | |||
| 12ng/ml | 1.088 | 1.075 | 1.032 |
| 1.062 | |||
| 6ng/ml | 0.415 | 0.432 | 0.389 |
| 0.448 | |||
| 3ng/ml | 0.297 | 0.255 | 0.212 |
| 0.212 | |||
| 1.5ng/ml | 0.097 | 0.108 | 0.065 |
| 0.118 | |||
| 0ng/ml | 0.036 | 0.043 | 0 |
| 0.049 |
Precision
We measured random samples of E5589Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 8.92 | 0.36 | 4.05 |
| Intra-Assay | 2 | 18 | 18.89 | 0.75 | 3.95 |
| Intra-Assay | 3 | 18 | 6.37 | 0.23 | 3.55 |
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