Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5592Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.143 | 2.093 | 2.036 |
| 2.043 | |||
| 800ng/L | 1.428 | 1.467 | 1.409 |
| 1.506 | |||
| 400ng/L | 0.767 | 0.774 | 0.717 |
| 0.781 | |||
| 200ng/L | 0.546 | 0.527 | 0.47 |
| 0.508 | |||
| 100ng/L | 0.263 | 0.257 | 0.199 |
| 0.25 | |||
| 0ng/L | 0.06 | 0.057 | 0 |
| 0.054 |
Precision
We measured random samples of E5592Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 932.58 | 31.16 | 3.34 |
| Intra-Assay | 2 | 18 | 351.43 | 19.34 | 5.5 |
| Intra-Assay | 3 | 18 | 302.87 | 17.65 | 5.83 |
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