Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5613Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.172 | 2.191 | 2.124 |
| 2.209 | |||
| 800ng/L | 1.471 | 1.427 | 1.36 |
| 1.382 | |||
| 400ng/L | 0.842 | 0.796 | 0.73 |
| 0.751 | |||
| 200ng/L | 0.406 | 0.432 | 0.365 |
| 0.457 | |||
| 100ng/L | 0.313 | 0.303 | 0.236 |
| 0.293 | |||
| 0ng/L | 0.068 | 0.067 | 0 |
| 0.066 |
Precision
We measured random samples of E5613Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 347.3 | 17.77 | 5.12 |
| Intra-Assay | 2 | 18 | 796.52 | 25.05 | 3.15 |
| Intra-Assay | 3 | 18 | 415.59 | 19.55 | 4.7 |
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