Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5631Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 2.172 | 2.223 | 2.184 |
| 2.273 | |||
| 800ng/L | 1.507 | 1.626 | 1.588 |
| 1.745 | |||
| 400ng/L | 1.086 | 1.099 | 1.06 |
| 1.112 | |||
| 200ng/L | 0.672 | 0.677 | 0.638 |
| 0.682 | |||
| 100ng/L | 0.429 | 0.421 | 0.383 |
| 0.413 | |||
| 0ng/L | 0.033 | 0.038 | 0 |
| 0.044 |
Precision
We measured random samples of E5631Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 664.01 | 33.75 | 5.08 |
| Intra-Assay | 2 | 18 | 121.52 | 7.74 | 6.37 |
| Intra-Assay | 3 | 18 | 362.48 | 22.91 | 6.32 |
Protocols
References
Customer reviews and Q&As
Associated products
