Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5635Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.556 | 2.531 | 2.484 |
| 2.506 | |||
| 1200ng/L | 1.461 | 1.442 | 1.395 |
| 1.424 | |||
| 600ng/L | 0.866 | 0.9 | 0.853 |
| 0.935 | |||
| 300ng/L | 0.53 | 0.493 | 0.446 |
| 0.456 | |||
| 150ng/L | 0.286 | 0.274 | 0.227 |
| 0.262 | |||
| 0ng/L | 0.031 | 0.047 | 0 |
| 0.063 |
Precision
We measured random samples of E5635Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 878.55 | 49.88 | 5.68 |
| Intra-Assay | 2 | 18 | 1326.41 | 39.01 | 2.94 |
| Intra-Assay | 3 | 18 | 234.91 | 14.97 | 6.37 |
Protocols
References
Customer reviews and Q&As
