Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5637Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.162 | 2.084 | 2.04 |
| 2.007 | |||
| 12ng/ml | 1.145 | 1.148 | 1.104 |
| 1.152 | |||
| 6ng/ml | 0.436 | 0.428 | 0.383 |
| 0.42 | |||
| 3ng/ml | 0.238 | 0.244 | 0.199 |
| 0.249 | |||
| 1.5ng/ml | 0.13 | 0.117 | 0.072 |
| 0.103 | |||
| 0ng/ml | 0.043 | 0.045 | 0 |
| 0.046 |
Precision
We measured random samples of E5637Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 15.07 | 0.4 | 2.68 |
| Intra-Assay | 2 | 18 | 10.83 | 0.41 | 3.77 |
| Intra-Assay | 3 | 18 | 5.52 | 0.18 | 3.33 |
Protocols
References
Customer reviews and Q&As
Associated products
