Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5639Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 4800ng/L | 2.07 | 2.178 | 2.132 |
| 2.286 | |||
| 2400ng/L | 1.537 | 1.593 | 1.546 |
| 1.648 | |||
| 1200ng/L | 1.024 | 1.081 | 1.035 |
| 1.138 | |||
| 600ng/L | 0.739 | 0.683 | 0.636 |
| 0.626 | |||
| 300ng/L | 0.44 | 0.466 | 0.42 |
| 0.492 | |||
| 0ng/L | 0.05 | 0.046 | 0 |
| 0.042 |
Precision
We measured random samples of E5639Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 487.89 | 39.2 | 8.03 |
| Intra-Assay | 2 | 18 | 1550.6 | 75.63 | 4.88 |
| Intra-Assay | 3 | 18 | 932.78 | 60.68 | 6.51 |
Protocols
References
Customer reviews and Q&As
Associated products
